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Code of Federal Regulations (Last Updated: May 6, 2024) |
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Title 9 - Animals and Animal Products |
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Chapter I - Animal and Plant Health Inspection Service, Department of Agriculture |
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SubChapter G - Livestock Improvement |
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Part 147 - Auxiliary Provisions on National Poultry Improvement Plan |
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Subpart B - Bacteriological Examination Procedure |
§ 147.17 - Laboratory procedure recommended for the bacteriological examination of cull chicks and poults for salmonella.
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Link to an amendment published at 79 FR 38766, July 9, 2014.
The laboratory procedure described in this section is recommended for the bacteriological examination of cull chicks from egg-type and meat-type chicken flocks and waterfowl, exhibition poultry, and game bird flocks and poults from turkey flocks for salmonella.
(a) For cull chicks, from 25 randomly selected 1- to 5-day-old chicks that have not been placed in a brooding house, prepare 5 organ pools, 5 yolk pools, and 5 intestinal tissue pools as follows. For poults, from a sample of 10 poults that died within 10 days after hatching, prepare organ pools, yolk pools, and intestinal pools as follows:
(1) Organ pool: From each of five chicks or two poults, composite and mince 1- to 2-gram samples of heart, lung, liver, and spleen tissues. Include the proximal wall of the bursa of Fabricius for chicks only.
(2) Yolk pool: From each of five chicks or two poults, composite and mince 1- to 2-gram samples of the unabsorbed yolk sac or, if the yolk sac is essentially absent, the entire yolk stalk remnant.
(3) Intestinal pool: From each of five chicks or two poults, composite and mince approximately 0.5 cm2 sections of the crop wall and 5-mm-long sections of the duodenum, cecum, and ileocecal junction.
(b) Transfer each pool to tetrathionate selective enrichment broth (Hajna or Mueller-Kauffmann) at a ratio of 1 part tissue pool to 10 parts broth.
(c) For cull chicks, repeat the steps in paragraphs (a) and (b) of this section for each 5-chick group until all 25 chicks have been examined, producing a total of 15 pools (5 organ, 5 yolk, and 5 intestinal). For poults, repeat the steps in paragraphs (a) and (b) of this section for each two-poult group until all the poults in the sample have been examined.
(d) Culture the tetrathionate pools as outlined for selective enrichment in illustration 2 of §147.11. Incubate the organ and yolk pools for 24 hours at 37 °C and the intestinal pools at 41.5 °C. Plate as described in illustration 2 of §147.11 and examine after both 24 and 48 hours of incubation. Confirm suspect colonies as described. Further culture all salmonella-negative tetrathionate broths by delayed secondary enrichment procedures described for environmental, organ, and intestinal samples in illustration 2 of §147.11. A colony lift assay may also be utilized as a supplement to TSI and LI agar picks of suspect colonies.
[61 FR 11525, Mar. 21, 1996, as amended at 72 FR 1425, Jan. 12, 2007]