2024-26232. Movement of Organisms Modified or Produced Through Genetic Engineering; Notice of Additional Modifications Exempt Plants Can Contain

Table 1—Summary of Modifications and Applicability to Polyploids

Notes	Designation	Modification
1 pair of homologous chromosomes	§ 340.1(b)(1)	The genetic modification is a change resulting from cellular repair of a targeted DNA break in the absence of an externally provided repair template.
1 pair of homologous chromosomes	§ 340.1(b)(2)	The genetic modification is a targeted single base pair substitution.
1 pair of homologous chromosomes	§ 340.1(b)(3)	The genetic modification introduces a gene known to occur in the plant's gene pool or makes changes in a targeted sequence to correspond to a known allele of such a gene or to a known structural variation present in the gene pool.
1 pair of homologous chromosomes across subgenomes without repair template and one pair of homologous chromosomes with repair template	340.1(b)(4)(vi)(AM1)	An indel or contiguous deletion of any size, made at a targeted location, with or without insertion of DNA if generated without using a repair template, or without insertion of DNA if generated using a repair template.
( print page 89582)
Allows up to 12 simultaneous (multiplex) or sequential modifications	340.1(b)(4)(vi)(AM2)	Plants with up to 12 modifications, made simultaneously or sequentially, are exempt from regulation if each modification individually qualifies the plant for exemption and occurs in a different gene. Modifications to either a single allele or pair of alleles on homologous chromosomes will count as one modification. See website for information on counting modifications.