96-10384. Standard Operating Procedure for Measuring Cholinesterases in Laboratory Rats and Dogs Exposed to Non-Reversible Cholinesterase Inhibitors

[Federal Register Volume 61, Number 82 (Friday, April 26, 1996)]
[Notices]
[Page 18593]
From the Federal Register Online via the Government Publishing Office [www.gpo.gov]
[FR Doc No: 96-10384]



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ENVIRONMENTAL PROTECTION AGENCY
[OPP-00432; FRL-5364-5]


Standard Operating Procedure for Measuring Cholinesterases in 
Laboratory Rats and Dogs Exposed to Non-Reversible Cholinesterase 
Inhibitors

AGENCY: Environmental Protection Agency (EPA).

ACTION: Notice of availability and request for comments.

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SUMMARY: EPA is making available for public comment Standard Operating 
Procedures (SOP) for measuring cholinesterases. This SOP specifies the 
details of a clinical methodology for performing cholinesterase enzyme 
assays in tissues taken from laboratory rats and dogs. This SOP is 
limited in scope to the measurement of cholinesterase levels in control 
animals and in animals exposed to a non-reversible cholinesterase 
inhibitor. This method is essentially the Ellman procedure (1961), with 
the stipulation that specific parameters be tightly controlled (e.g. 
pH, wavelength, substrate concentration, etc.).

DATES: Written comments must be received on or before May 28, 1996..

ADDRESSES: Interested persons are invited to submit written comments in 
triplicate to: Public Response and Program Resources Branch, Field 
Operations Division (7506C), Environmental Protection Agency, 401 M 
St., SW., Washington, DC 20460. In person: Bring comments to: Rm. 1132, 
CM #2, 1921 Jefferson Davis Highway, Arlington, VA.
    Comments and data may also be submitted electronically by sending 
electronic mail (e-mail) to: [e-mail]. Electronic 
comments must be submitted as an ASCII file avoiding the use of special 
characters and any form of encryption. Comments and data will also be 
accepted on disks in WordPerfect in 5.1 file format or ASCII file 
format. All comments and data in electronic form must be identified by 
the docket number ``OPP-00432.'' Confidential Business Information 
(CBI) should be submitted through e-mail. Electronic comments on this 
document may be filed online at many Federal Depository Libraries. 
Additional information on electronic submissions can be found under 
``SUPPLEMENTARY INFORMATION.''
    Information submitted as a comment in response to this notice may 
be claimed confidential by marking any part or all of that information 
as CBI. Information so marked will not be disclosed except in 
accordance with procedures set forth in 40 CFR part 2. A copy of the 
comment that does not contain CBI must be submitted for inclusion in 
the public docket. Information not marked confidential will be included 
in the public docket without prior notice.

FOR FURTHER INFORMATION CONTACT: By mail: Robert B. Jaeger, Designated 
Federal Official, FIFRA Scientific Advisory Panel (7509C), Office of 
Pesticide Programs, U.S. Environmental Protection Agency, 401 M Street, 
SW., Washington, DC 20460. Office location and telephone number: Rm. 
819B, CM #2, 1921 Jefferson Davis Highway, Arlington, VA [phone] 
or 305-7351; e-mail: [e-mail].
    Copies of the SOP may be obtained by contacting: By mail: Public 
Docket and Freedom of Information Section, Field Operations Division 
(7506C), Office of Pesticide Programs, Environmental Protection Agency, 
401 M St., SW., Washington, DC 20460. In person or for courier pick-up: 
Office location and telephone number: Rm. 1132, CM #2, 1921 Jefferson 
Davis Highway, Arlington, VA, [phone] or 305-5454. By internet: 
e-mail requests to: [e-mail]. The SOP is also 
electronically available on EPA's gopher server (gopher://
gopher.epa.gov) and the world wide web (www)     (http://www.epa.gov) 
under the heading ``Rules, Regulations and Legislation''.

SUPPLEMENTARY INFORMATION: A public record has been established for 
this notice under docket number ``OPP-00432'' (FRL-5364-5) (including 
comments and data submitted electronically as described below). A 
public version of this record, including printed, paper versions of 
electronic comments, which does not include any informaiton claimed as 
CBI, is available for inspection from 8:00 a.m. to 4:30 p.m., Monday 
through Friday, excluding legal holidays. The public record is located 
in Rm. 1132, Public Response and Program Resources Branch, Field 
Operations Division (7506C), Office of Pesticide Programs, 
Environmental Protection Agency, Crystal Mall #2, 1921 Jefferson Davis 
Highway, Arlington, VA.
    Electronic comments can be sent directly to EPA at:
    [e-mail]
    Electronic comments must be submitted as an ASCII file avoiding the 
use of special characters and any form of encryption.
    The official record for this notice, as well as the public version, 
as described above will be kept in paper form. Accordingly, EPA will 
transfer all comments received electronically into printed, paper form 
as they are received and will place the paper copies in the official 
record which will also include all comments submitted directly in 
writing. The official record is the paper record maintained at the 
address in ``ADDRESSES'' at the beginning of this document.

List of Subjects

    Environmental protection.

    Dated: April 18, 1996.

Stephanie R. Irene,
Acting Director, Health Effects Division, Office of Pesticide Programs.

[FR Doc. 96-10384 Filed 4-25-96; 8:45 am]
BILLING CODE 6560-50-F